Introduction
Metabolic Solutions offers project design assistance
and a mass spectrometry service to measure CO2 to
help researchers determine substrate oxidation in
studies using stable isotope tracers.
The oxidation of carbon-13 labeled tracers can be
quantitated using breath or blood carbon dioxide.
We have extensive experience in measuring 13C
enrichment in CO2 for oxidation studies.
List of Substrate Oxidation Services
- Breath Tests
- Breath 13CO2
- Blood 13CO2
Published Oxidation Studies Analyzed By Metabolic Solutions
1. Lariviere, F., R. Moussalli, D.R. Garrel.
Increased leucine flux and leucine oxidation during
the luteal phase of the menstrual cycle in women.
Am. J. Physiol. 267(30):E422-E428, 1994.
"Possible
changes in protein metabolism during the menstrual cycle
were examined in eight healthy women who received an
intravenous infusion of L-[1-13C]leucine one time during
the follicular phase of the menstrual cycle and one
time during the luteal phase. Enrichment of plasma
[13C]ketoisocaproate and expired 13CO2
were measured to determine leutine flux and oxidation."
2. Caprio, S., Cline, G., Boulware, S., Permanente,
C., Shulman, G.I., Sherwin, R.S., Tamborlane, W.V.
Effects of puberty and diabetes on metabolism of insulin-sensitive
fuels. Am J. Physiol. 266(29):E885-E891, 1995.
"Insulin's ability to stimulate glucose metabolism
is reduced during normal puberty; these changes are
exaggerated in adolescents with insulin-dependent diabetes
mellitus (IDDM). Because the effects of puberty
and IDDM on the other actions of insulin have not been
established, we studied leucine kinetics (using [1-13C]leucine)
and fat metabolism during euglycemic hyperinsulinemia
for 3 h in eight healthy and nine IDDM adolescents and
six healthy young adult controls."
3. Stadtlander, C.T.K.-H, Stutzenberger, F.J.
Adaptation of the [13C]Urea breath test as
a noninvasive method for detection of Helicobacter pylori
infection in squirrel monkeys (Saimiri spp.).
Laboratory Animal Science, 45(3):239-243, 1995.
"The [13C]urea breath test was
adapted for use in squirrel monkeys (Saimiri spp.) for
identification of experimentally induced infection with
Helicobacter pylori, the bacterium causing gastric ulcer
in humans."
4. Slomianski, A., Schubert, T., Cutler, A. [13C]Urea
Breath Test to confirm eradication of Helicobacter pylori.
Am J Gastro, 90(2):224-226, 1995.
"To determine the utility of the [13C]urea
breath test in confirming the eradication of Helicobacter
pylori."
5. Webb, D.D., Ciociola, A.A., Perschy, T.B.,
Goodwin, B.B. Effect of Administration of Ranitidine
Bismuth Citrate with Food on the suppression and eradication
of Helicobacter pylori in infected volunteers. Am J
Gastro, 90(8):1273-1277, 1995.
"This study examined the effect of administration
with food on the ability of ranitidine bismuth citrate
to suppress and eradicate Helicobacter pylori in 41
infected volunteers."
6. Cutler, A.F., Havstad, S., Ma, C.K., Blaser, M.J.,
Perez-Perez, G.I., Schubert, T.T., Accuracy of invasive
and noninvasive tests to diagnose Helicobacter pylori
infection. Gastroenterology 109:136-141, 1995.
"Our aim was to compare the sensitivity, specificity,
and negative and positive predictive value of the most
widely available tests for diagnosis of H. pylori.
A total of 268 patients (mean age, 53.7 +/- 15.8 years;
142 male and 126 female; 125 white and 143 nonwhite)
was tested for H. pylori infection by [13C]urea breath
test (UBT), measurement of serum immunoglobulin (lg)
G and IgA antibody levels, and antral biopsy specimens
for ClO test, histology, and Warthin-Starry stain."
7. Liebman, M., Chai, W. Effect of dietary calcium
on urinary oxalate excretion after oxalate loads. Am
J Clin Nutr 65:1453-1459, 1997.
"An experimental
model that allowed differentiation between endogenously
and exogenously derived urinary oxalate was used to
assess the effect of different forms and doses of ingested
calcium on oxalate absorption and excretion."
8. Borel, M.J., Buchowski, M.S., Turner, E.A., Peeler,
B.B., Goldstein, R.E., Flakoll, P.J., Alterations in
basal nutrient metabolism increase resting energy expenditure
in sickle cell disease., Am. J. Physiol. 274(37):E357-E364,
1998.
"Basal rates of whole body protein,
glucose, and lipid metabolism and resting energy expenditure
(REE) were measured in eight African-American sickle
cell disease (SCD) patients and in six African-American
controls."
9. Friedlander, A.L., Casazza, G.A., Horning, M.A.,
Buddinger, T.F., Brooks, G.A., Effects of exercise intensity
and training on lipid metabolism in young women. Am.
J. Physiol. 275(38):E853-E863, 1998.
"We
examined the effects of exercise intensity and training
[12 wk, 5 days/wk, 1 h, 75% peak oxygen consumption
(VO2peak)] on lipolysis and plasma free fatty acid (FFA)
flux in women (n =8; 24.3 +/- 1.6yr)."
10. Liebman, M., Costa, G. Effects of calcium
and magnesium on urinary oxalate excretion after oxalate
loads. J Urol 163(5):1565-1569, May 2000.
"The primary objective of this study was to
compare the effects of calcium carbonate and magnesium
oxide on oxalate absorption."
11. Trimmer, J.K., Casazza, G.A., Horning, M.A., Brooks,
G.A. Autoregulation of glucose production in men
with a glycerol load during rest and exercise.
Am J Physiol Endocrinol Metabl. 280(4):E657-E668, 2001.
"Related to hepatic autoregulation we evaluated
hypotheses that 1) glucose production would be altered
as a result of a glycerol load, 2) decreased glucose
recycling rate (Rr) would result from increased glycerol
uptake, and 3) the absolute rate of gluconeogenesis
(GNG) from glycerol would be positively correlated to
glycerol rate of disappearance (R(d)) during a glycerol
load."
12. Trimmer, J.K., Casazza, G.A., Horning, M.A., Brooks.
Recovery of 13CO2 during rest
and exercise after [1-13C]acetate, [2-13C]acetate,
and NaH13CO3 infusions. Am J Physiol
Endocrinol Metabl. 281(4):E683-E692, 2001.
"Fractional recoveries of 13C label in respiratory
gases were determined during 225 min of rest and 90
min of leg cycle ergometry at 45 and 65% peak oxygen
uptake (VO2 peak) after continuous infusions
of [1-13C]acetate, or NaH13CO3."
13. Ikizler, A., Pupim, L.B., Brouillette, J.R., Levenhagen,
D.K., Farmer, K., Hakim, R.M., Flakoll, P.J., Hemodialysis
stimulates muscle and whole body protein loss and alters
substrate oxidation. Am J Physiol Endocrinol Metab 282:E107-E116,
2002.
"The hemodialysis (HD) procedure
has been implicated as a potential catabolic factor
predisposing the chronic HD (CHD) patients to protein
calorie malnutrition. To asses the potential effects
of HD on protein and energy metabolism, we studied 11
CHD patients 2 h before, during and 2 h after HD by
use of primed constant infusion of L-[1-13C]leucine
and L-[ring-2H5]phenylalanine."
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