Metabolic Solutions offers project
design assistance and a mass spectrometry service to
help researchers study nitrate synthesis using stable
isotope methods.
Endogenous nitric oxide has a wide biological role in
modulating physiological and pathophysiological processes.
Nitric oxide is synthesized from the amino acid L-arginine
by the enzyme nitric oxide synthase. In the presence
of biological tissues, nitric oxide is rapidly converted
to nitrate. Nitric oxide production can be estimated
from urinary nitrate excretion. Nitric oxide synthesis
rates can be measured by administering the stable isotope
15N2-guanido-arginine and observing
the immediate enrichment of 15N in nitrate.
List of Nitrate/Nitric Oxide Metabolism Services:
- Endogenous nitrate synthesis
- Nitric oxide precursor (arginine) kinetics
Published Nitrate Studies Analyzed By Metabolic
Solutions, Inc.
1. Hibbs, J.B., Westenfeider, C., Taintor, R., Vavrin,
Z., Kablitz, C., Baranowski, R.L., Ward, J.H., Menlove,
R.L., McMurry, M.P., Kushner, J.P., Samlowski, W.E.
Evidence for cytokine-inducible nitric oxide synthesis
from L-Arginine in patients receiving interleukin-2
therapy. J. Clin. Invest. 89:867-877, 1992.
"We examined renal cell carcinoma (RCC; n=5)
and malignant melanoma (MM; n=7) patients for evidence
of cytokine-inducible NO synthesis. Activity
of this pathway was evaluated by measuring serum and
urine nitrate (the stable degradation product of NO)
during IL-2 therapy."
2. Katz, S.D., Khan, T., Zeballos, G.A., Mathew, L.,
Potharlanka, P., Knecht, M. Whelan, J. Decreased
activity of the L-arginine-nitric oxide metabolic pathway
in patients with congestive heart failure. Circulation,
April 27:99(16):2113-2117, 1999.
"To specifically
assess the synthetic activity of the L-arginine-NO metabolic
pathway, urinary excretion of [15N]nitrates and
[15N]urea was determined after a primed after a primed
continuous intravenous infusion of L-[15N]arginine (40micromol/kg)
in 16 patients with congestive heart failure and 9 age-matched
normal control subjects at rest and during submaximal
treadmill exercise."
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