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Stable Isotope Analytical Services

Metabolic Solutions offers a service for the measurement and analysis of stable isotopes (13C, deuterium oxide, 15N, oxygen 18) in a state-of-the-art mass spectrometry and HPLC laboratory. Since 1990, Metabolic Solutions has provided stable isotope measurements to over five hundred researchers worldwide. Included among our clients are most of the top 50 pharmaceutical companies (see client list). These clients recognize Metabolic Solutions for its strict compliance to current Good Laboratory Practices and trust us to produce the quality of work necessary for submission to regulatory agencies like the Food and Drug Administration.

The following are some of the most common analytical services we provide:

Isotope Ratio Mass Spectrometry (IRMS)

Stable Isotope Analysis	Substrate	Optimal sample volume required	Minimum sample volume required	Precision of the Instrument
¹³CO₂	CO₂ Gas or Blood	2-5% in 10 ml Exetainer tube*	0.5% in 10 ml Exetainer tube*	0.2 SD
Deuterium (D₂O)	Body Fluids	1 ml	50 µl single measurement	2% SD
H₂O-18	Body Fluids	0.5 ml	50 µl	0.4 SD
¹⁵N Combustion	Solids & dissolved solids	10 mg N	0.1 mg N in combustion capsule	Instrument at 0.4 SD, depending on homogeneity
¹⁵N Ammonia Combustion	Urine	2 ml	1 ml	Instrument at 0.4 SD
¹⁵N Urea Combustion	Urine & Plasma	2 ml	1 ml	Instrument at 0.4 SD
¹³C Combustion	Solids & dissolved solids, some liquids possible	10 mg C	0.1 mg C in combustion capsule	Instrument at 0.4 SD, depending on homogeneity
¹³C Glucose Combustion	Plasma	2 ml	0.5 ml one shot	Instrument at 0.2 SD

Gas Chromatography/Mass Spectrometry (GC/MS)

Analysis	Substrate	Optimal sample volume required	Minimum sample volume required	Precision of the Instrument at Optimal Levels
¹³CGlucose	Plasma	300 µl	50 µl	0.2 % SD
6,6 D₂Glucose	Plasma	300 µl	50 µl	0.2 % SD
D₅Glycerol	Plasma	500 µl	100 µl	0.2 % SD
¹³CKIC	Plasma	400 µl	100 µl	0.2 % SD
¹³CLeucine	Plasma	400 µl	100 µl	0.2 % SD
¹⁵NNitrate	Plasma	1000 µl	500 µl	0.2 % SD

High Performance Liquid Chromatography (HPLC)

Analysis	Substrate	Optimal sample volume required	Minimum sample volume required	Precision of the Instrument
Sodium Bromide (NaBr)	Body Fluids	200 µl	75 µl	5% SD

Liquid Scintillation Counting

Analysis	Substrate	Optimal sample volume required	Minimum sample volume required	Precision of the Instrument
¹⁴CO₂	Breath	2 mmol of CO₂	1 mmol of CO₂	Chi-square determined daily using unquenched standards.

* Exetainer tubes for storing gas specimens are available by calling Metabolic
Solutions (603) 598-6960.

Metabolic Solutions, Inc., 460 Amherst Street, Nashua, NH 03063

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Key Industry terms: total body water, body composition, deuterium oxide, sodium bromide, extracellular water, , energy expenditure, doubly labeled water, oxygen 18, gastric emptying, breath test, amino acid metabolism, lipid metabolism, protein turnover, glucose metabolism, substrate oxidation