Metabolic Solutions offers project
design assistance and a mass spectrometry service to
help researchers to study amino acid metabolism using
stable isotope methods.
The quantification of various aspects of specific amino
acid metabolism in human subjects is achieved with stable
isotope tracers. Initially, protein and amino acid nutrition
was based on the nitrogen (N) balance technique. However,
it is widely accepted that a major portion of body protein
is continuously degraded and resynthesized, but remains
in N balance. Stable isotope techniques can dissect
the relative contributions of protein synthesis and
degradation in relation to body N balance. Furthermore,
stable labeled amino acid tracers can be utilized to
determine how amino acid metabolism is affected by various
nutritional and non-nutritional factors.
List of Amino Acid Metabolism Services:
- Metabolic fate of dietary amino acids
- Amino acid kinetics for requirement studies
- Oxidation
- Transamination
- Extracellular intermediates
Published Amino Acid Studies Analyzed By Metabolic Solutions
1. Long, C.L., Nelson, K.M., DiRienzo, D.B., Weis, J.K.,
Stahl, R.D., Broussard, T.D., Theus, W.L., Clark, J.A.,
Pinson, T.W., Geiger, J.W., Laws, H.L., Blakemore, W.S.,
Carraway, R.P. Glutamine Supplementation of Enteral
Nutrition: Impact on whole body protein kinetics and
glucose metabolism in critically i11 patients.
Journal of Parenteral and Enteral Nutrition 19(6):470-476,
1995.
"We investigated the effect
of an enteral formula on protein and glucose kinetics
and nitrogen balance in trauma patients. After
3 days of feeding, protein kinetics were measured using
a 4-hour prime continuous infusion of L-[1-13C]leucine."
2. Cincotta, A.H., MacEachern, T.A., Meier, A.H. Bromocriptine
redirects metabolism and prevents seasonal onset of
obese hyperinsulinemic state in Syrian hamsters. Am.
J. Physiol. 264(27):E285-E293, 1993.
"Metabolic
and hormonal effects of bromocriptine were studied in
seasonally obese female Syrian hamsters. 15N-lysine
flux and deamination was measured after 10 wks of treatment."
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